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The main reason that a doctor would prescribe vitamin B12 injections is if the patient is unable to obtain enough vitamin B12 on their own, whether they have pernicious anemia or are unable to get enough of the vitamin through their diet alone
Regular injections help replenish these levels and sustain overall health
Reported applications include NNMT enzyme inhibition assays measuring 1-methylnicotinamide formation or S-adenosylhomocysteine release, intracellular target-engagement assays in differentiated 3T3-L1 adipocytes where 5-Amino-1MQ reduced intracellular 1-methylnicotinamide with an EC near 2.3 M (Neelakantan et al., Biochem Pharmacol, 2018), membrane permeability and Caco-2 transport characterisation, methyltransferase selectivity panels covering COMT, DNMT1 and PRMT3, and rodent studies of NNMT inhibition in adipose tissue and liver following the antisense knockdown work of Kraus and colleagues (Nature, 2014)
Pernicious anemia happens when people develop an autoimmune condition that reduces the production of intrinsic factor
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