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Additionally, T1 also shows a better de-pigmenting effect than kojic acidat 50 M, kojic acid decreases 12.9% pigmentation level, while T1 can decrease 3-fold of that (41.4%), as shown in Fig

The culture medium was removed, and the cells were incubated with serum-free DMEM containing 10 M DCFH-DA at 37 C for 20 min