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After digestion, the peptides in each sample were desalted on C18 cartridges, concentrated by vacuum centrifugation and reconstituted in 40 l of 0.1% (v/v) trifluoroacetic acid

The reconstitution calculator determines exact water volumes for target concentrations

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By adding 4mL, the concentration is equalized to the 10mg/1mL scenario, making dose volumes familiar for the researcher

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