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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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agent model), there are prostaglandins analoguesethanol model (Robert, 1979) relationships, NSAIDsacetic acid model (Okabe and Amagase, 2005) relationships, dopamine agonistscysteamine model (Selye and Szabo, 1973) relationships, and H 2 blockerscysteamine model (Selye and Szabo, 1973) relationships

It is more accurate to call it a dual agonist that includes GLP-1 activity rather than a pure GLP-1 medication