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Decreased IIS in nonhuman organisms has been associated with extended lifespan and protection against oxidative stress-mediated age-dependent damage [5, 6]
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The tissue was homogenized in 4 volumes of 5 mM phosphate buffer, pH 7.4 and centrifuged at 10,000 g for 15 min to obtain supernatant which used for the assay of antioxidant profile and protein determination of the animals.[] Analytical procedures Antioxidant enzymes assay The activity of superoxide dismutase (SOD) was measured using a method determined by Sun et al .[] The activity of CAT was measured using a method determined by Aebi.[] Malondialdehyde determination The concentration of malondialdehyde (MDA) in the homogenate of the liver was measured using a method determined by Ohkawa et al .[] as thiobarbituric acid reactive substances
Measure against baseline weekly
Traxler L, Herdy JR, Stefanoni D, Eichhorner S, Pelucchi S, Szcs A, et al
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