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Antibody-stained protein signal was amplified and visualized using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher #34578) and imaged with a ChemiDoc MP Imaging system (Bio-Rad)

SIBO is formally defined as a bacterial population in the small intestine exceeding 1010 colony-forming units (CFU) per milliliter, though the exact diagnostic threshold remains debated

Zinc, when included, also contributes to immune cell development

However, if you are new to using these active ingredients, you may want to alternate their applications to minimize the chance of irritation