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Subsequently, adapter trimming was performed using Cutadapt (v1.14), and low-quality reads were removed with Trimmomatic (v0.36)

Li Y, Zhu R, Liu Y, et al

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Eluted protein samples were filtered and injected into an autosampler-equipped KTA pure system on a Superdex S75 Increase 10/300 GL column at room temperature using SEC running buffer (20 mM Tris-HCl and 100 mM NaCl (pH 8))

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Keep in mind that excessive alcohol consumption and binge drinking can be dangerous