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2020;2020(98):S1115
Example 19 preparation of Semetreuptade 59.4 g of the peptide resin obtained in example 15 was added to a 2000ml single-neck flask, and lysate 600ml of TFA was prepared in advance: and (3) TIS: EDT (electro-thermal transfer coating): PhOH: H2O 90:3:3:2:2 (vol.%), the lysate was added to the flask, the reaction was carried out at room temperature for 2.5 hours, the resin was filtered off, the resin was washed with 50ml tfa, the filtrates were combined, 6000ml of anhydrous ether was added to precipitate a white solid, the solid was centrifuged, the solid was washed with anhydrous ether, and 20.2 g of crude semaglutide peptide was dried in vacuo to a white solid, with a yield of 98.24%
Lifestyle Integration GLP-1s and peptides work best when combined with nutrition, fitness, and hydration strategies
The supernatant was reconstituted using 50% v/v acetonitrile in water and centrifuged again
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