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In one of these studies, measuring brain GSH levels with in vivo proton MRS yielded a correlation in GSH reduction and decline in cognitive functions with 87.5% sensitivity and 100% specificity (Mandal et al., 2015)
Extraction and enzymes assays Fresh maize leaves (1 g) were homogenized in 100 mM Tris-HCl in presence of 5 mM DTT (dithiothreitol), 10 mM MgCl 2 , 1 mM EDTA, 5 mM magnesium acetate, 1.5% polyvinylpyrrolidone (PVP-40), 1 mM phenylmethylsulfonyl fluoride, and 1 g mL 1 aprotinin
Because heavy metals increase oxidation in the body, chelation decreases oxidative stress
Additionally, there is an elevation in blood glucose and uric acid production
Heavy metals, such as lead, mercury, and cadmium, can deplete glutathione levels, impairing the body's ability to detoxify harmful substances and reducing the availability of SAMe for methylation
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