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Weight regain usually happens for a few common reasons: Returning to old eating habits Not getting enough protein Losing muscle during weight loss Stopping exercise routines Depending only on appetite suppression Stopping medication too quickly Some people regain most of the weight, while others maintain their results successfully

Techniques such as meditation, deep breathing exercises, and yoga can help manage stress

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Then, the plates were incubated for 72 h in a CO2 incubator under appropriate culture conditions