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After drug treatments, the cells were fixed with 4% paraformaldehyde for 20 min and infiltrated with 0.2% Triton X-100 for 15 min, followed by blocking in donkey serum for 30 min and incubating overnight at 4C with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS
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A structural explanation for these findings was provided from our previous cryo-EM structures of CT(Pro32) and rAmy(Tyr37)-bound AMYRs structures where the bulky phenolic side chain of Y37 rAmy forms perpendicular stacking interactions with W79 ECD 15 , and additional interactions with the respective RAMP protomer that are likely important for selectivity of rAmy for AMYRs over CTR
In the normal renal function group, one participant had a clinically significant vital sign abnormality (increased blood pressure) and one had a clinically significant ECG abnormality, with both reported as resolved mild TEAEs
The research team then calculated the wet/dry ratio of each specimen in order to compare them to the values of the control groups
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