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Cell suspensions were serially diluted and plated on TSA with 0.4% activated charcoal (Sigma) to neutralize residual antibiotics, allowing enumeration of surviving bacteria
10.3390/genes14040933 162 PangM.WangS.ShiT.ChenJ
Timing optimization also reduces side effects
Dealing with Precipitation Issues What it looks like: visible particles or haze forming after the peptide seemed dissolved, usually a pH or solvent mismatch
The environment should remain quiet throughout the test
Monotherapy protocols are commonly initiated at 0.6 mg per week and escalated to a maximum studied dose of 4.5 mg as tolerated
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