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After 48 h, the mice were sacrificed, and the heart, liver, spleen, lung, kidney, and tumor tissues were subsequently isolated, weighed, and nitrated for further analysis

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Given that EtOH-induced mitochondrial dysfunction and ER stress are intimately involved in the pathogenesis of ALD, future studies to examine these pathways under the current experimental setting will provide important mechanistic information underlying the protection seen in GCLM KO mice

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