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The transfection complex was formed at a DNA:PEI ratio of 1:3 in OPTI-MEM (Life Technologies, Paisley, UK), with a 30 minute incubation at room temperature prior to addition to the cells
Temporal adjustment of the juxtaglomerular apparatus during sustained inhibition of proximal reabsorption
Vitamin C Supports collagen production and overall skin health
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Resources Gandhi, Guneet, et al