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The mice lung tissue stored in liquid nitrogen were homogenized in ice-cold 50 mM TrisHCl buffer (pH 7.55)

Studies in mice with CNS neuron-specific GLP-1R knockout showed that liraglutide failed to induce weight loss but still improved glucose levels, suggesting that brain GLP-1Rs are necessary for the weight-lowering effect of GLP-1RAs but not essential for their glucose-lowering effects (142)

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Inadequate initial treatment may result in suboptimal responses and persistent symptoms

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