When cells reached 80% confluence, the monolayer was rinsed 3x with PBS and cultured in phenol red-free MEM without additives (no FBS, L-glutamine, ITS, or estradiol)
They are oral, regulated as supplements rather than injectables, and considered low risk
1 Introduction Osteoarthritis (OA) is among the most prevalent degenerative joint diseases globally, affecting more than 500 million people worldwide and ranking as the leading cause of disability among adults aged 50 years and older (1, 2)
Free Rad Biol Med 4 :185198
Moreover, retinal I/R injury was shown in animal models by transient elevation of intraocular pressure and a mechanistic increase in ROS, and this injury was reversed by the continuous delivery of H 2 saturated eye drops, especially in apoptosis (Oharazawa et al., 2010)
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