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Titration of GRX1-roGFP2 and roGFP2 was done with DTT, which has a more negative reduction potential than lipoic acid ( E DTT = 323 mV, Shaked et al., Plant material and growth conditions Heterozygous Arabidopsis rml1 mutants were selected by genotyping for the mutant allele and exploiting the fact that the point mutation (Vernoux et al., rml1 seedlings were selected for further propagation
A study by Forsling et al
Full results may take up to 3 months
Reconstitution technique also plays a role
Processing of cholesterol and protein gives propionyl-CoA that is converted to methylmalonyl-CoA, which is used by MUT enzyme to make succinyl-CoA
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