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Restricted rotation around amide bonds may influence conformational equilibrium in solution, observable through advanced spectroscopic techniques

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Blue N, Methylene 4

The accumulation of NADH leads to a reduction in the coenzyme Q pool, and coupled with the malateaspartate shuttle, a surplus of fumarate accumulates and is then converted to succinate by the reverse catalysis of succinate dehydrogenase (SDH) [73, 74]