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For the Triacsin C treated group, 1 M of the inhibitor was added together with azide-PA and cultured for 24 h
The pituitary gland and hypothalamus were collected and frozen for analysis of mRNA expression of GH, GHRH, GHSR, SST, and SSTR by real-time polymerase chain reaction (RT-PCR) and protein expression by Western immunoblotting
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