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Cysteine is the rate-limiting factor in cellular GSH synthesis, since this amino acid is relatively rare in foods
Sample preparation for T cell proteomics A375 melanoma tumor cells (ATCC, CRL-1619) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillinstreptomycin (VWR, 16777-164) at 37 C under 5% CO 2
