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Purity testing typically uses High Performance Liquid Chromatography to identify the peptide and assess the concentration of the target compound versus impurities
For the targeted sulfur metabolite quantification approach, the previously established LC-MS conditions were applied 99 with selected reaction monitoring (MRM) using an Ultimate 3000 UPLC system coupled to a Thermo Finnigan TSQ Quantum equipped with HESI (Thermo Fisher Scientific, Waltham, MA)
Major published semaglutide trials Trial STEP 1 (Wilding et al., NEJM 2021) Design Phase 3 / 68 weeks Population 1,961 adults with obesity, no diabetes Key result -14.9% body weight (semaglutide 2.4 mg) vs -2.4% placebo
Standards of Medical Care in Diabetes2021 abridged for primary care providers
I would also like to thank David L Ludwig for valuable discussions about the physiology and pharmacology of GLP-1 and for careful reading and commenting on the manuscript
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