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Calzada, E., Onguka, O

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Next, the FLAG-UBQLN2/anti-FLAG beads were washed twice with BA100 buffer and three times with LLPS buffer before being incubated with HA-UBQLN2 recombinant protein for 2 h at 4 C with constant rotation

Because SDH is the only enzyme that participates in both the TCA cycle and the ETC, we speculated that the ETC might be compromised in Gclc -deficient FoB, thus explaining the inhibition of SDH and the accumulation of succinate