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Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb
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The dogs were acclimatized to the housing conditions for at least 7 days prior to the initiation of the experiment
Vitamin C 1,000 mg two to three times daily (Stengler 2016) 1000 mg per day of ascorbic acid for two months reduced cortisol in women with functional hypercortisolemia (Begyarlan 2023) Dehydroepiandrosterone (DHEA) 25 to 200 mg/d (Kroboth 2003
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