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When AD293 cells reached 7080% confluence, 50 ng GLP-1R TMD (WT or C347K) plasmid were co-transfected with 50 ng membrane-tethered ECD-peptide/ECD fusion plasmid, 200 ng of the CRE-luciferase reporter construct and 10 ng of the TKRenilla construct using Lipofectamine 2000 reagent (1:2)

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The fruit were divided randomly into three replicates per species, each consisting of six samples

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When necessary, effective but strong prescription medications can also be called upon