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In addition to being an amino acid, glutamate is a neurotransmitter, the major excitatory neurotransmitter of the vertebrate central nervous system. Glutamate in the brain is either synthesized in the brain or obtained from the recycling of brain proteins. Levels of glutamate in the brain (10,00012,000 M) are at least 100 times higher than circulating blood levels (50100 M). Because of the blood-brain barrier (which prevents many substances in the bloodstream from entering the brain), levels of glutamate in the blood do not affect levels of glutamate in the brain. Under certain pathological conditions (e.g., ischemic stroke), release of glutamate from brain cells can occur, leading to extreme excitation of nerve cells

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After 24 h, fresh cell media with or without 5FU was applied to the cells and the cells were treated for a further 72 h

These findings align with existing literature on the diagnostic and prognostic implications of acyl-carnitines, which have shown that increased plasma levels of these lipid compounds are associated with the occurrence or burden of chronic conditions such as renal failure, AF, or diabetes 35,36,37

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