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(4) Activated PLC then possibly breaks down a specific component of the cell membrane into two signaling molecules, inositol trisphosphate (IP) and diacylglycerol (DAG)
Remove any air bubbles by tapping the syringe and gently pressing the plunger until a small droplet appears at the needle tip
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In addition, palmitate inhibits the phosphorylation of adenosine 5denosinelation_ENREF_57 protein kinase (AMPK) and blocks autophagy, leading to increased ROS levels in macrophages, which in turn activate the NLRP3 inflammasome and IL-1 secretion (58)
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