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10.1016/j.cell.2018.02.007 Cell 282 TorresJ
Either the peptide needs a different solvent (like acetic acid water) or it's degraded
This injection should be administered only if advised by a physician
Using the wrong diluent
After mixing the sample or standard with the detection reagent and incubating at room temperature for 15 minutes, the fluorescent product is read at 510 nm in a fluorescent plate reader with excitation at 390 nm
doi: 10.2147/JEP.S35396
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