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Molecular Biotechnology
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(10) Engel MG, Narayan S, Cui MH, Branch CA, Zhang X, Gandy SE, Ehrlich M, Huffman DM
Cell culture, treatment, and transfection Human HEK293T and HT1080 cells were obtained from the American Type Culture Collection (ATCC) and maintained (37 C, 5% CO 2 ) in DMEM high-glucose medium (Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), penicillinstreptomycin (100 U/mL, Gibco, 15140148), sodium pyruvate (1 mM, Gibco, 11360070), and MEM nonessential amino acids (1, Gibco, 11140050)
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