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PDK4 can upregulate RUNX2 and ALP activity, increase calcium deposition and calcified nodule formation, and promote the transformation of VSMC osteogenesis [89]
Encouragingly, experiments show that antioxidant interventions in aged animals (including administering melatonin or vitamin E) can restore some amplitude to circadian rhythms and improve activity/rest pattern stability (Reiter et al., 2024)
The deeper anti-aging effects, epigenetic, antioxidant, immune, are measured over a full cycle and beyond, best assessed through objective follow-up data rather than subjective experience alone
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Treb RM (2018)
J.JannK.et al
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