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When you stop a drug for a period of time and then attempt to re-measure the outcome, this may give you a glimpse or a clue as to whether the drug delayed disease progression
This can lead to uncomfortable constipation and even abdominal pain in some cases
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Regarding duloxetine, a review by Crucitti et al
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cAMP accumulation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were diluted to the density of 6 10 5 cells/ml and INS-1(832/13) cells to 2 10 6 cells/ml in stimulation buffer [phenol-free DMEM containing 5 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) and 0.1% (w/v) BSA], and cAMP detection was carried out as described previously 48
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