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c , HCC-1806 cells were grown in control (208 M cystine

To obtain a sufficient level of methionine auxotrophy, in all the figures presented (except Figure 1B, in which 2 centromeric plasmids were used), CYS4 overexpression was obtained through the transfection of two 2 vectors of the pRS42X series and the addition in the medium of serine (at a final concentration of 1.5 mM), a limiting substrate for Cys4p activity

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