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The soluble clarified fraction was recovered by centrifuging at 75,000 g for 20 min at 4 C and applied to a Superdex 200 26/600 column (Cytiva) for SEC, which was run as above

For individuals with sensitive or acne-prone skin, these harsh treatments can exacerbate acne by damaging the skins natural moisture barrier and increasing inflammation

Sitohang IBS, et al

Using higher doses or off-label products may increase the risk

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