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Blots were blocked with Odyssey blocking buffer (LiCore Biosciences) for 1 hr and then incubated in GPX4 antibody (AbFrontier) diluted 1:2000

The dosage used was 6150 g/kg intraperitoneally

These findings suggest that our peptide exhibits potent functionality within cells as a senolytic drug, with selectivity for senescent cells

DSIP differs in that it directly modulates neuropeptide and neurotransmitter pathways associated with sleep induction rather than acting primarily as a circadian signal