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The PI3K/Akt pathway activated by IGF1R is a major anti-apoptotic pathway in neurons, which drives interest in IGF1R agonism as a potential neuroprotective strategy in preclinical research

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These intestinal sections were flushed with ice-cold phosphate-buffered saline (PBS) to remove fecal matter and placed in 1- to 2-cm pieces into 15 mL sterile tubes with 5 mL dissociation buffer (PBS, 10 mM EDTA) for incubation at 37C in a shaker at 250 rpm for 20 minutes

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