2.3 Cisplatin induces mitochondrial dysfunction When cisplatin enters the mitochondria, it acts directly on the inner mitochondrial membrane, leading to the impairment of the function of the electron transport chain, the reduction of ATP production and the induction of ROS generation (Tan and Song, 2023),these damages further lead to mitochondrial dysfunction, which in turn prompts more ROS production, forming a positive feedback loop (Tan and Vlajkovic, 2023)
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Note: this work used full-length T4, not the TB-500 heptapeptide fragment
Using correct materials prevents contamination and maintains sterility throughout the procedure
Although reconstitution itself is only one step within the overall peptide handling process, it works together with proper storage, documentation, and laboratory technique to support reliable research outcomes