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S4), indicating a potential substitution of Sec by Cys at this site during evolution

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Huh7 and Hep3B cells were cultured in high-glucose Dulbeccos modified Eagles medium (DMEM, Huh7) and minimum essential medium (MEM, Hep3B), containing 10% fetal bovine serum (FBS) in a 37 C incubator with 5% CO 2