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For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, Cat# H-3300-250)
Months 2-3: Accelerating loss as doses increase
Medical Some of the medical impacts and complications associated with bulimia nervosa include: Chronic sore throat, indigestion, heartburn and reflux Inflammation and rupture of the oesophagus and stomach from frequent vomiting Stomach and intestinal ulcers Chronic irregular bowel movements, constipation and/or diarrhoea due to deliberate misuse of laxatives Heart problems including slow heart rate, irregular heartbeat and low blood pressure Electrolyte disturbance, including potassium and sodium Osteoporosis or osteopenia: a reduction in bone density caused by a specific nutritional deficiency Fatigue and lethargy Loss of or disturbance to menstruation Increased risk of infertility Death Psychological Some of the psychological impacts and complications associated with bulimia nervosa include: Extreme body dissatisfaction/distorted body image Obsessive thoughts and preoccupation with eating, food, body shape and weight Social withdrawal Feelings of shame, guilt, and self-loathing Depressive or anxious symptoms and behaviours Self-harm or suicidality Substance misuse Recovery It is possible to recover from bulimia nervosa, even if a person has been living with the illness for many years
For the public and healthcare system, it underscores the importance of careful prescribing practices, monitoring of drug use, and raising awareness about the potential risks associated with these medications
UV exposure will undo every effort within weeks
