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The vital dye 2-(4-(dimethylamino)styryl)- N -ethylpyridinium iodide (DASPEI) (Life Technologies, Grand Island, NY) is a marker of mitochondrial membrane potential and preferentially stains lateral line hair cells when added to the surrounding EM (Harris et al., 2000) per fish were assessed based on fluorescent intensity (Harris et al., Aminoglycoside uptake Gentamicin conjugated to the fluorophore Texas Red (GTTR) was used to determine if Dihexa affected aminoglycoside uptake by hair cells (Steyger et al., 2003)
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