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Briefly, recombinant GSTO1 was diluted into 50 mM Tris-HCl (pH 8.0) buffer containing the fluorophore 1,8 ANS (0.3 mM) to a final concentration of 0.3 mg ml 1

At this level the risk of copper staining or reactive irritation is low, and daily use is realistic

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And I realize that's a bit of a coarse way to approach it, but I feel fine and I'm still breathing and ambulatory