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[8] Chemistry [edit] Ionization [edit] When glutamic acid is dissolved in water, the amino group (NH 2 ) may gain a proton (H + ), and/or the carboxyl groups may lose protons, depending on the acidity of the medium

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The answer determines the protocol

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Two things follow: the chemical identity of what is in the well is no longer what was weighed out, and the oxidative stress generated can itself change transcription and proliferation in ways that look like compound activity