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Depending on the plate layout, either LPS (1 g/ml) or ConA, (2.5 g/ml, Sigma-Aldrich, Steinheim, Germany) was added in four replicates per cow in addition to four unstimulated wells (with Seahorse medium for volume compensation)
This condition is often accompanied by increased cortisol levels
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To draw a 200 mcg dose, the calculation is: (200 mcg 2,500 mcg/mL) = 0.08 mL, or 8 units on a standard U-100 insulin syringe
doi: 10.1016/j.exger.2009.07.003 125 HanJKaufmanRJ
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