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Both types of inhibitors can block the demethylase function, but scaffolding inhibitors also interfere with LSD1 in complex with other epigenetic regulators ( Our group has previously published that the scaffolding LSD1 inhibitor SP-2509 and its clinical successor SP-2577, or seclidemstat, potently suppress the viability and metabolism of NK cells ( Materials and Methods Primary Human Samples and Cell Line Culture Human ex vivo expanded NK cells were previously isolated from de-identified healthy donor peripheral blood mononuclear cells (PBMCs), expanded with feeder cells, and cryopreserved as stocks in liquid N 2 ( Chemicals and Reagents LSD1 inhibitors tranylcypromine (TCP) (Enzo Biosciences), GSK LSD1 (Cayman Chemical), RN-1 (Cayman Chemical), SP-2509 (Cayman Chemical), and SP-2577 (kindly provided by Salarius Pharmaceuticals) were reconstituted in DMSO or PBS (TCP) and aliquoted for storage at 20C

OConnor, C

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