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JAMA Network Open, 7(5), e2413172

Eight healthy volunteers were given a calorie-restricted diet with either oral MK-677 or a placebo for two 14-day periods

After centrifugation at 15,700 g for 5 min the supernatant was discarded and the cell pellet was repeatedly washed in 5 mL of TE buffer and resuspended in 3 mL of lithium/caesium acetate solution (Qbiogene)