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Therefore, the differences in these proteins could explain the reduced expression of GshF in the mutant ZJ12345 compared to the wild type strain and help explain why the mutant required a higher IPTG concentration to obtain GshF activity similar to the wild type host strain

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According to a 2017 review published in American Family Physician (AAFP), the prevalence of vitamin B12 deficiency in the United States and the United Kingdom is approximately 6% in persons younger than 60 years, and nearly 20% in those older than 60 years

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