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For assessment of glucose-induced insulin secretion, culture medium was removed and islet microtissues were equilibrated for 1 h with Krebs Ringer Hepes Buffer (KRHB
It was shown that iron chelator (deferoxamine) and certain antioxidants explicitly inhibit this type of cell death
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Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated
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Participants switched to placebo after the run-in regained, with a body weight change of +6.9% from week 20 to week 68, versus 7.9% in those who continued
