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Colorectal cancer cells (HCT116 and MC38) were initially cultured in folate-deficient medium for 2 days, then transferred to media containing varying folate concentrations (20 nM and 200 nM) for 2 days, after which tumor supernatants and cells were harvested

Bioavailability after subcutaneous injection is assumed, not proven

The Pearson correlation coefficient r is approximately 0.836, indicating a strong negative correlation between vitamin B12 levels and HDRS scores