2019 Aug;1866(8):1272-81.
10.1523/JNEUROSCI.3178-06.2006 15
Evidence comparison: GHK-Cu has a substantially larger body of published research, spanning from Pickart's original 1973 isolation through hundreds of subsequent studies
Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)
Joint Stiffness or Discomfort Elevation of GH may result in temporary joint stiffness as the tissues are adapting to the increased regenerative activity, particularly during the first few weeks
Research shows that while 50-60% of users experience gastrointestinal side effects initially, these are usually mild to moderate and transient