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To further confirm that the endogenous carnitine shuttle provides an acetyl-CoA pool that can be used for histone acetylation, we conducted 13 C 6 -glucose tracing in cells with knockdown of the carnitine acetyltransferases CrAT and CrOT

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The membranes were incubated with primary antibodies at 4C overnight

These may include: A vitamin B12 deficiency can also lead to pernicious anemia

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