After further culturing for 24 h, each well was washed, 1 mg mL 1 MTT solution (100 L) was added, and the cells were re-incubated for 4 h in the dark
If the solution looks green, teal, or any color other than blue, something has gone wrong
Plant Physiol 142:13641379 Noshi M, Yamada H, Hatanaka R, Tanabe N, Tamoi M, Shigeoka S (2017) Arabidopsis dehydroascorbate reductase 1 and 2 modulate redox states of ascorbateglutathione cycle in the cytosol in response to photooxidative stress
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What they found: No serious adverse events triggered by AOD-9604 The most frequent side effects: headache (~5 %), occasional fatigue, at the same frequency as placebo No changes in IGF-1 levels, glycemia, blood pressure, heart rate No signals of cardiovascular, hepatic or renal toxicity Safety profile comparable to placebo Why it matters: From a safety perspective, AOD-9604 is one of the safest peptides in the clinical registry