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For the peptide 20GCGRG s complex, cell pellets were resuspended in 20 mM HEPES, pH 7.4, 50 mM NaCl, 2 mM MgCl 2 with protease inhibitor cocktail, EDTA-free, 5 M peptide 20, 10 g/mL Nb35 and 25 mU/mL apyrase

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The clinical status of the cows in the present study also shows a pro-inflammatory and anti-inflammatory period, according to the gene expression profile of PBMC

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